Fig. 1
From: NMDAR mediated translation at the synapse is regulated by MOV10 and FMRP
Effect of NMDAR stimulation on MOV10 interaction with AGO2 and distribution in polysomes. a Immunoblots for MOV10 and AGO2 after AGO2-immunoprecipitation on NMDAR stimulation. Quantitative analysis of MOV10 association with AGO2 following AGO2 immunoprecipitation on NMDAR stimulation in rat cortical synaptoneurosomes (n = 5, paired Student’s t-test, ±SEM). Values are normalized to basal levels. b Distribution of RPLP0 on linear sucrose gradient from rat cortical synaptoneurosomes after cycloheximide or puromycin treatment based on immunoblots shown below. c Distribution of MOV10 on linear sucrose gradient from rat cortical synaptoneurosomes after cycloheximide or puromycin treatment based on immunoblots shown below (representative of three experiments, also see Additional file 1: Figure S2A). d Quantification of MOV10 in heavy polysomes (fraction 7–11) for cycloheximide or puromycin treatment (n = 3, paired Student’s t-test, ±SEM). Values are normalised to cycloheximide levels. e Distribution of MOV10 on linear sucrose gradient from rat cortical synaptoneurosomes after NMDAR stimulation based on immunoblots shown below (representative of five experiments, also see Additional file 1: Figure S2B). f Quantification of MOV10 in heavy polysomes (fraction 7–11) for NMDAR stimulation as compared to basal condition (n = 5, paired Student’s t-test, ±SEM). Values are normalised to basal levels