Fig. 2
From: NMDAR mediated translation at the synapse is regulated by MOV10 and FMRP
MOV10 interaction with AGO2 and its distribution in polysomes requires FMRP. a Immunoblots for MOV10 and AGO2 for input and after AGO2-immunoprecipitation on fmr1 knockdown in Neuro2a cells. Quantitative analysis of MOV10 association with AGO2 following AGO2 immunoprecipitation from Neuro 2a cells transfected with scramble siRNA (scr-siRNA) or fmr1 siRNA (fmr1-siRNA) (n = 4, unpaired Student’s t-test, ±SEM). b Immunoblots for MOV10 and AGO2 after AGO2-immunoprecipitation from wild type (WT) or Fmr1-KO synaptoneurosomes (WT and Fmr1-KO samples were run on separate immunoblots). Quantitative analysis of MOV10 association with AGO2 following AGO2 immunoprecipitation from WT or Fmr1-KO synaptoneurosomes (n = 3, unpaired Student’s t-test, ±SEM). c Immunoblots for MOV10 after sucrose step gradient on fmr1 knockdown in Neuro2a cells. Quantification of MOV10 in 30% fraction (heavy polysomes) separated on a sucrose step gradient from Neuro 2a cells transfected with scramble siRNA (scr-siRNA) or fmr1 siRNA (fmr1-siRNA). Values normalised to samples from scrambled siRNA transfected cells (n = 3, unpaired Student’s t-test, ±SEM). d Immunoblots of ribosomal protein RPLP0 and MOV10 on a linear sucrose gradient from wild-type (WT) or Fmr1 KO rat cortical synaptoneurosomes. e Immunoblots of MOV10 on a linear sucrose gradient from wild-type (WT) and Fmr1 KO rat cortical synaptoneurosomes on NMDAR stimulation. Distribution of MOV10 on linear sucrose gradient from Fmr1-KO rat synaptoneurosomes on NMDAR stimulation based on adjacent immunoblots