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Fig. 5 | Molecular Brain

Fig. 5

From: NMDAR mediated translation at the synapse is regulated by MOV10 and FMRP

Fig. 5

NMDAR stimulation leads to translational upregulation of MOV10-FMRP target mRNAs. a Ribosome distribution on linear sucrose gradient on cycloheximide and puromycin treatment based on RPLP0 immunoblot (below) from rat cortical synaptoneurosomes. b, c Quantitative distribution of RPLP0 protein or Psd-95 mRNA on linear sucrose gradient cycloheximide and puromycin treatment normalized to cycloheximide levels (n = 3, unpaired Student’s t test, ±SEM). d-g Distribution of mRNAs on linear sucrose gradient from synaptoneurosomes at basal state and on NMDAR stimulation followed by quantification of mRNAs in polysomes (bar graphs, n = 3, paired Student’s t-test, ±SEM) for the mRNAs; Pten (d), Psd-95 (e), Ank2 (f) and b-actin (g). Also see Additional file 1: Figure S6C-S6F. h Immunoblot showing PTEN protein after NMDAR stimulation with or without AP-5. Quantification of PTEN levels on NMDAR stimulation normalized to Tuj1 (n = 6, one way ANOVA, Tukey’s multiple comparison test, ±SEM). i Immunoblot showing PSD-95 protein after NMDAR stimulation with or without AP-5. Quantification of PSD-95 levels on NMDAR stimulation normalized to Tuj1 (n = 4, one way ANOVA, Tukey’s multiple comparison test, ±SEM)

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