Skip to main content
Fig. 5 | Molecular Brain

Fig. 5

From: Altered cortical Cytoarchitecture in the Fmr1 knockout mouse

Fig. 5

FMRP-deficient astrocytes become reactive and display actin reorganization. a Primary astrocyte cultures derived from WT and Fmr1 KO neonatal brains are immunostained with GFAP. b KO-astrocytes have a pronounced increase in GFAP fluorescent intensity and astrocyte surface area, which are characteristics of reactive astrogliosis (n = 30–34 per group). c Western blot analysis confirms that GFAP expression is substantially higher in Fmr1 KO astrocytes (n = 4 different cultures per group). d Representative fluorescent images showing F-actin fibres (labeled with phalloidin) in GFAP-astrocyte cultures. Normal astrocytes display a distinct well-organized evenly distributed pattern, but F-actin in FMRP-depleted astrocytes form clusters in the cytoplasmic region. Scale Bar: 20 μm. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01

Back to article page