Fig. 2

Functional effects of Rabconnectin-3 on Cav2.2 channels expressed in tsA-201 cells. (a) Representative set of current traces recorded in response to depolarizing steps ranging from − 50 mV to 40 mV from a holding potential of − 80 mV for cells expressing Cav2.2 channels with RB-3α or RB-3β or both. (b) Current density-voltage relationships for cells expressing Cav2.2 channels with RB-3α or RB-3β. Inset. Corresponding mean half activation potential. (c) Corresponding maximal conductance. Numbers shown in the bars reflect numbers of cells (d) Steady-state inactivation curves for cells expressing Cav2.2 channels with RB-3α or RB-3β. Inset. Corresponding mean half activation potential. (e) Proteins from tsA-201 cells transfected with Cav2.2α₁ and RB-3β were immunoprecipitated with anti-Cav2.2, anti-RB-3β or control (Irr) antibodies and followed by Western blot analysis using anti-RB-3β. (f) Quantification of biotinylation experiments from tsA-201 cells transfected with Cav2.2/β₁/α₂δ-1 with and without RB-3β. Biotinylated cell surface protein was isolated and normalized to Na/K-ATPase levels. Numbers shown in the bars reflects numbers of independent experiments. (g) Current density-voltage relationships for cells expressing Cav2.2 channels with RB-3(α + β). (h) Steady-state inactivation curves for cells expressing Cav2.2 channels with RB-3(α + β).