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Fig. 4 | Molecular Brain

Fig. 4

From: Disruption of Foxg1 impairs neural plasticity leading to social and cognitive behavioral defects

Fig. 4

Foxg1 elimination led to reduced dendritic complexity and spine density. (a-b′) Undetectable changes in hippocampal structure between WT and cKO viewed by DAPI staining (a’ and b′, High magnification views of boxed CA1 regions in A and B, respectively. Scale bar, 200 μm). c Cell counting of boxed CA1 regions in A’ and B′ showed comparable cell numbers (WT, 6 slices from 3 mice; cKO, 6 slices from 3 mice, P = 0.2795; t test). d cKO mice displayed an obvious reduction in dendritic branches in hippocampal CA1 pyramidal neurons as viewed by Golgi staining (Scale bar, 50 μm). e Total dendritic length was significantly reduced in cKO mice (WT, 37 cells from 4 mice; cKO, 25 cells from 4 mice, P < 0.0001; t test). f cKO mice exhibited reduced dendritic complexity in both apical and basal dendrites of hippocampal CA1 pyramidal neurons (WT, 35 cells from 4 mice; cKO, 32 cells from 4 mice, F (54, 3575) = 2.568, P < 0.0001; two-way ANOVA, distance x group, repeated measure). g Representative images of spines from apical secondary branches (Scale bar, 2.5 μm). h Spine density was significantly reduced in cKO mice (WT, 54 cells from 4 mice; cKO, 35 cells from 4 mice, P = 0.0002; t test). i-j Post hoc staining of biocytin showed a strong reduction in dendritic branches (i) and spines (j) in cKO neurons (Scale bars, i: 200 μm; j: 20 μm). ***P < 0.001, ****P < 0.0001

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