Fig. 5
From: Disruption of Foxg1 impairs neural plasticity leading to social and cognitive behavioral defects
Loss of Foxg1 led to impairment in dendritic complexity and axonal maintenance. (a-c′) Immunostaining of anti-Map 2 in cultured primary neurons at DIV 7 (a, a’) and DIV 14 (b-c′) exhibited decreased dendritic branches in Foxg1-deficient neurons (Scale bar, 100 μm). d Application of Sholl analysis showed decreased dendritic complexity at DIV 14 in Foxg1-deficient neurons (WT n = 30, cKO n = 22, F (39, 1950) = 3.085, P < 0.0001; two-way ANOVA, distance x group, repeated measure). e Both maximum and total dendritic length were reduced in Foxg1-deficient neurons (WT, n = 30; cKO, n = 20; Maximum length, P = 0.0021; Total length, P < 0.0001; t test). f-g’ Foxg1-deficient neurons displayed a shorter axon at DIV 5 as viewed by immunostaining of anti-Tau (Scale bar, 50 μm). h Quantification of axonal length revealed significantly reduced axonal length in Foxg1-deficient neurons (WT, n = 57; cKO, n = 42, P < 0.0001; t test). Data was from three repeated cell culture experiments, **P < 0.01, ****P < 0.0001