Fig. 2

Altered layer-specific markers in LIMK1 KO and LIMK2 KO cortex. a, b Sample coronal brain sections of postnatal day 0 (P0) stained for the late-born neuronal marker Brn2 (layer II and III) and summary graph showing reduced Brn2⁺ neurons in LIMK2 KO and LIMK1/2 DKO mice. c and d Sample coronal brain sections stained for the layer V neuronal marker Ctip2 and summary graph showing reduced Ctip2⁺ neurons in LIMK1/2 DKO mice. e and f Sample coronal brain sections stained for the layer VI neuronal marker Tbr1 and summary graph showing a trend of reduction in Tbr1⁺ neurons in LIMK2 KO and LIMK1/2 DKO mice. WT n = 7, LIMK1 KO n = 6, LIMK2 KO n = 6, LIMK1/2 DKO n = 5. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA, Dunnett’s test. Scale bars, 50 μm