Fig. 6

Coronal brain sections of WT control and LIMK KO mice E14.5 embryos were stained for Pax6 or Tbr2. a Pax6 (red) and DAPI (blue) staining of WT control and various LIMK KO mice. b Summary graph of Pax6 positive cells showing no differences between control and various LIMK KO mice. c Tbr2 (green) and DAPI (blue) staining of WT control and various LIMK KO mice. d Summary graph showing significantly decreased numbers of Tbr2 positive cells in LIMK1 KO, LIMK2 KO and DKO mice compared to their control littermates. Control, n = 6; LIMK1 KO, n = 3; LIMK2 KO, n = 5; LIMK1/2 DKO, n = 3. Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA, Dunnett’s test. *p < 0.05, **p < 0.01. Scale bars, 50 μm