Fig. 3
From: Postsynaptic densities fragment into subcomplexes upon sonication
Sonication promotes dissociation of cytoskeletal elements (c&d) while major PSD constituents (b&e) are recovered in the pellet. Three types of samples, control (C), sonicated (S) and sonicated+ (S+, additional sonication at output amplitude setting of 100%), were prepared and centrifuged as detailed in Methods. Supernatants and pellets corresponding to 10 μg original protein were separated by SDS-PAGE and Western immunoblots were obtained using indicated antibodies