Fig. 2

Microglial P2Y12R deficiency enhances innate, but not learned fear responses. a Elevated Plus Maze (EPM) evaluation of anxiety between constitutive microglial P2Y12R KO (n = 19) mice and WT controls (n = 21). b The constitutive KO mice spent less time in the open arms and were less likely to enter into the open arms than control mice. c Light/dark box testing evaluation of anxiety between constitutive KO mice (n = 15) and WT controls (n = 19). d Constitutive KO mice spent less time exploring the lighted side and stepped out less from the dark box. e Protocol for fear conditioning training and tests. Mice were foot shocked at the last 2 s of a 30 s auditory tone, the tone and foot shock pair were repeated for 3 times. Freezing responses to the context and tone stimulus were measured in the next day. f The microglial P2Y12R KO mice showed similar freezing responses to the training context and paired auditory tone stimulus as the WT control mice (n = 17 for the WT control, n = 11 for the constitutive KO group). **p < 0.01, ***p < 0.001, t-test and U-test. Data are presented as the mean ± SEM