Fig. 1

Endogenous USP5 SUMOylation levels in sham operated mice and in mice with a sciatic nerve injury. a. USP5 immunoprecipitates reveal that USP5 is SUMOylated, as seen by western blots probed against SUMO 2/3.Furthermore, there is a decrease in SUMO2/3 signal in ipsilateral DRGs from SNI mice when compared to ipsilateral DRGs from Sham mice, b. USP5 immunoprecipitates show increased USP5 levels in ipsilateral DRGs from SNI mice when compared to ipsilateral DRGs from Sham mice c. Protein control using an α-Tubulin antibody to probe DRG tissue from ipsilateral Sham or ipsilateral SNI mice. d. Densitometry analysis of USP5 SUMO 2/3 signals from USP5 immunoprecipitates normalized to USP5 expression levels. Numbers in parentheses reflect numbers of mice, the asterisk denotes statistical significance at the 0.05 level