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Fig. 1 | Molecular Brain

Fig. 1

From: Ankyrin B and Ankyrin B variants differentially modulate intracellular and surface Cav2.1 levels

Fig. 1

AnkB mutations increased expression of Cav2.1 without altering its binding affinity. (ai) Representative Western blots of whole cell lysates of HEK293T cells transfected with Cav2.1 and GFP control or wildtype or mutant AnkB-GFP. (aii) Quantification of Cav2.1 normalized to GAPDH and expressed as a percentage of control (CTL). One-way ANOVA followed by Sidak’s multiple comparison, N = 5, F (4, 20) = 15.59, p < 0.0001. (aiii) Quantification of GFP normalized to GAPDH and expressed as a percentage of wildtype. One-way ANOVA, N = 5, F (3, 16) = 1.408, p = 0.2770. (bi) Representative co-IP Western blots of transfected HEK293T cells. (bii) Quantification of Cav2.1 co-IP normalized to GFP IP and Cav2.1 input and expressed as a percentage of wildtype. One-way ANOVA, N = 3, F (3, 8) = 0.9381, p = 0.4662. This data is included in the MSc thesis of CSWC, University of Victoria, 2019 found at https://dspace.library.uvic.ca//handle/1828/11053

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