Fig. 6
From: Ankyrin B and Ankyrin B variants differentially modulate intracellular and surface Cav2.1 levels
Decreased Cav2.1 expression levels in whole cortex homogenate of P30 AnkBglut+/−. (a) Flow chart of synaptosome preparation procedure. (bi) Representative Western blots of P14 and P30 homogenate (H) and synaptosome (Syn) fraction from control (CTL; AnkBwt/wt: Emx1IRES-cre) and AnkB conditional heterozygous knockout (AnkBglut+/−; AnkBflox/wt: Emx1IRES-cre) cortices. (bii) Quantification of immunoblots normalized to stain-free and expressed as a percentage of control homogenate. Two-way ANOVA followed by Sidak’s multiple comparison, N = 5, interaction F (1, 16) = 4.047, p = 0.0614, genotype: F (1, 16) = 59.15, p < 0.0001, fraction F (1, 16) = 332.4, p < 0.0001 (P14 AnkB); N = 5, interaction F (1, 16) = 0.7049, p = 0.4135, genotype: F (1, 16) = 68.89, p < 0.0001, fraction F (1, 16) = 85.56, p < 0.0001 (P30 AnkB); N = 5, interaction F (1, 16) = 0.0269, p = 0.8716, genotype: F (1, 16) = 3.025, p = 0.1012, fraction F (1, 16) = 143.3, p < 0.0001 (P14 Cav2.1); N = 5, interaction F (1, 16) = 10.47, p = 0.0052, genotype: F (1, 16) = 0.6942, p = 0.4170, fraction F (1, 16) = 181.7, p < 0.0001 (P30 Cav2.1); N = 5, interaction F (1, 16) = 0.4101, p = 0.5310, genotype: F (1, 16) = 0.1144, p = 0.7396, fraction F (1, 16) = 29.99, p < 0.0001 (P14 syntaxin 1A); N = 5, interaction F (1, 16) = 3.163, p = 0.0943, genotype: F (1, 16) = 6.345, p = 0.0228, fraction F (1, 16) = 34.34, p < 0.0001 (P30 syntaxin 1A); N = 4, interaction F (1, 12) = 2.869, p = 0.1161, genotype: F (1, 12) = 0.013, p = 0.9108, fraction F (1, 12) = 514.9, p < 0.0001 (P14 PSD95); N = 4, interaction F (1, 12) = 11.33, p = 0.0056, genotype: F (1, 12) = 0.2076, p = 0.6568, fraction F (1, 12) = 117.8, p < 0.0001 (P30 PSD95). This data is included in the MSc thesis of CSWC, University of Victoria, 2019 found at https://dspace.library.uvic.ca//handle/1828/11053