Fig. 2

F1 reduces Aβ plaques in the cortex of APP/PS1 mice. 12-month-old APP/PS1 mice were orally administered F1 (20 mg/kg/day; n = 9) or vehicle (n = 6) for 8 wk. a 6E10-stained Aβ plaques in the hippocampal region (first row) and the retrosplenial cortex region (second row), red; ThS-stained Aβ plaques in the hippocampal region (third row) and the retrosplenail cortex (fourth row), green. b In the hippocampal region, the percentage of 6E10 positive plaque area (two-tailed Student’s t-test, t₍₁₃₎ = 0.8877, P = 0.3908), the density of 6E10 positive plaques (two-tailed Student’s t-test, t₍₁₃₎ = 0.7997, P = 0.4382), the percentage of ThS positive plaque area (two-tailed Student’s t-test, t₍₁₃₎ = 1.217, P = 0.2453), and the density of ThS positive plaques (two-tailed Student’s t-test, t₍₁₃₎ = 2.065, P = 0.0594); (c) In the retrosplenial cortex region, the percentage of 6E10 positive plaque area (two-tailed Student’s t-test, t₍₁₃₎ = 0.4549, P = 0.6567), the density of 6E10 positive plaques (two-tailed Student’s t-test, t₍₁₃₎ = 0.7706, P = 0.4547), the percentage of ThS positive plaque area (two-tailed Student’s t-test, t₍₁₃₎ = 2.238, P = 0.04334), and the density of ThS positive plaques (two-tailed Student’s t-test, t₍₁₃₎ = 2.298, P = 0.0388); The brain schematic diagram [39] shows the brain region of hippocampal and retrosplenial cortex images. n values indicate the number of mice. Data are mean ± s.e.m. *P < 0.05