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Fig. 3 | Molecular Brain

Fig. 3

From: Expression of the RNA methyltransferase Nsun5 is essential for developing cerebral cortex

Fig. 3

Dynamic expression of Nsun5 during corticogenesis. a & b Bar graphs show the levels of cerebral cortex Nsun5 mRNA and Nsun5 protein in E10.5, E12.5, E14.5, E16.5, E18.5, PND1 (P1) and PND10 (P10) WT mice, and E14.5 Nsun5-KO mice (KO). *P < 0.05 and **P < 0.01 vs. E10.5 mice (one-way ANOVA). c Representative images of double immunostaining in cerebral cortices from WT mice for (c) Nsun5 (red) and nestin (green) at E14.5, Scale bars, 25 μm; d Sox2 (green) and Nsun5 (red) at E14.5, Scale bars, 25 μm (views of entire cortex); Scale bars, 25 μm (in VZ); e Nsun5 (red) and Tbr2 (green) at E16.5, Scale bars, 25 μm (views of entire cortex); Scale bars, 25 μm (in SVZ); f Nsun5 (green) and Ctip2 (red) at E18.5, Scale bars, 25 μm (views of entire cortex); Scale bars, 25 μm (in CP); g Nsun5 (red) and Satb2 (green) at E18.5, Scale bars, 25 μm (views of entire cortex); Scale bars, 25 μm (in CP). Open arrows represent Nsun5+/nestin+ basal processes of RGC (c); arrows indicate Sox2+ cells (d), Tbr2+ cells (e), Ctip2+ cells (f) and Satb2+ cells (g)

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