Fig. 1

TNBS-treated rats exhibit abdomen hyperalgesia and anxiety-like behaviors. a-c: Representative pancreas histology of sham group (a), TNBS-treated group on POD 14 (b) and POD 28 (C). Bar = 100 μm. d-f: TNBS treatment increased serum content of amylase (d), lipase (e) and total bilirubin (f) compared to sham rats along the course of CP, one-way ANOVA in (d, e) and Kruskal-Wallis test in (f). g: TNBS-treated rats showed decreased abdomen withdrawal threshold along the course of CP, while rats with sham surgery exhibited transient abdomen mechanical hypersensitivity which returned to baseline on POD 14, one-way repeated ANOVA. h-i: Rats with CP traveled less distance in the open field (h) as well as in the center of the open field (i) than sham rats from POD 7 to 28, unpaired t-test. Numbers within the bars denote the numbers of rats in each group, * P < 0.05, ** P < 0.01, *** P < 0.001, TNBS vs sham; ^### P < 0.001, sham vs naïve