Fig. 10
From: Anterior insular cortex mediates hyperalgesia induced by chronic pancreatitis in rats
Chemogenetic inactivation of bilateral insular pyramidal neurons alleviates abdomen mechanical hyperalgesia and anxiety-like behavior in CP rats. a: The schematic diagram of the behavioral experiment (up) and rAAV2/9-CaMKIIa-hM4Di-mCherry construct (down). b: A representative coronal section showing virus injection sites within bilateral aIC. Bar = 1 mm. c: Inhibiting bilateral insular pyramidal neurons via i.p. CNO injection significantly increased the AWT in CP rats on POD 14. d, e: Inhibiting bilateral insular neurons led to a significant increase in total traveling distance (d) as well as central traveling distance (e) in CP rats. n = 6 in each group, unpaired t-test. * P < 0.05, ** P < 0.01, *** P < 0.001, Gi vs mCherry