Table 1 Antibodies used immunohistochemical staining
From: Anterior insular cortex mediates hyperalgesia induced by chronic pancreatitis in rats
Antigens | Primary antibodies | Secondary antibodies | Tertiary antibodies |
|---|---|---|---|
FOS | Mouse anti-FOS (1:500; Abcam, Cambridge, MA, USA) | Biotinylated donkey anti-mouse (1:500; Millipore, Billerica, MA, USA) | Avidin-biotin complex (1:200; ABC kit, Vector, Burlingame, CA, USA) |
VGluT1/NeuN | Mouse anti-NeuN (1:200; Millipore, Billerica, MA, USA) Rabbit anti-VGluT1 (1:300; Sysy, Gottingen, NI, GER) | Alexa594-donkey anti-mouse (1:500; Invitrogen, Carlsbad, CA, USA) Alexa488-donkey anti-rabbit (1:500; Invitrogen, Carlsbad, CA, USA) | Â |