Fig. 2
From: FGF receptors are required for proper axonal branch targeting in Drosophila
- Altered Btl expression results in axonal branch growth errors and misrouting. a-d Disrupted Btl expression produced a higher frequency of axonal branching errors. Expression of Btl dsRNA solely within the pSc neuron had a higher frequency of routing errors and absence of core branches (b), similar to Btlnull MARCM mutants (c). Btl RNAi and Btlnull mutants both showed significant decreases in total branch lengths. d Btl overexpression resulted in an increase in total branch length and significantly more ectopic axonal branches and routing errors. e Rescue expression of Btl in MARCM mutants reverted the axonal branching patterns to wildtype. Red indicates reduction of branches, green indicates ectopic branches. f Btl loss of function versus overexpression had opposing effects on axonal branch length (g) and number of branches (h). Scale bar, 50 μm. Arrowheads point to absent branches, arrows point to ectopic branches. Error bars are S.D.