Fig. 4
From: FGF receptors are required for proper axonal branch targeting in Drosophila
– Axonal targeting errors impair neuronal connectivity and mechanosensation. a Stimulation of the pSc bristle via application of fluorescent dye triggers a grooming response. A positive response was visually scored as the successful movement of the rear pair of legs onto the labelled bristles with spread of the dye to the rear legs. Scale bar is 1 mm. b, c Disrupting the pSc connectivity led to a significant decrease (p < 0.01) in response rate of all Btl and Htl mutant groups when compared to control. c Direct correlation of the pSc axonal branching pattern with the animal’s behavioral response revealed that animals with more severe axonal targeting errors (“no response”, far left and far right images) were more likely to be non-responsive to mechanical stimulation. Scale bar is 50 μm. Total branch lengths (d) and branch numbers (e) were significantly smaller in Btl RNAi responders (p < 0.05) and Btl RNAi non-responders (p < 0.01) compared to controls. Error bars are S.D.