Fig. 3

Increased lactate level detected in the hippocampus of streptozotocin-injected mice fed an HFD (HFD + STZ). a The amount of lactate in 40 μg of a lysate measured in the hippocampus. b LDHA and LDHB protein levels were measured by western blot assay. c LDHA and d LDHB protein levels were quantified by the ratio to β-actin in the hippocampus. e Amount of lactate in 40 μg of a lysate of the cortex. f Cropped images of LDHA and LDHB in the cortex. g Quantified LDHA and h LDHB in the cortex by the ratio to β-actin. Values are expressed as means ± SEMs (n = 5 for both groups). * p < 0.05