Fig. 5
From: Hyperpolarized [1-13C]lactate flux increased in the hippocampal region in diabetic mice
Phosphorylated ACLY (Ser455) increased in both the hippocampus and cortex in the streptozotocin-injected mice fed an HFD (HFD + STZ). a Expected metabolic pathway to supply pyruvate via ACLY. OAA; oxaloacetate, ME1; malic enzyme1, MDH; malate dehydrogenase, PEPCK; phosphoenolpyruvate carboxykinase, PK; pyruvate kinase, ACLY; ATP citrate lyase. b Phosphorylated ACLY (Ser455) and total ACLY protein levels were measured by western blot assay. Phosphorylated ACLY (Ser455) was quantified by the ratio of total ACLY; representative cropped images in the hippocampus and c in the cortex are presented. Values are expressed as means ± SEMs (n = 5 for both groups). * p < 0.05., ** p < 0.01