Table 1 PCR primers
From: Hyperpolarized [1-13C]lactate flux increased in the hippocampal region in diabetic mice
Gene | Â | Â | Cycle | size |
|---|---|---|---|---|
Glut1 | Forward | GGGTCTTAAGTGCGTCAGGG | 30 | 312 |
NM_011400.3 | Reverse | AGAGAGACCAAAGCGTGGTG | Â | Â |
Glut2 | Forward | ACCGGGATGATTGGCATGTT | 35 | 288 |
NM_031197.2 | Reverse | GAACACGTAAGGCCCAAGGA | Â | Â |
Glut3 | Forward | TGTGGTAAAAAGCAGGAGGGG | 35 | 251 |
NM_011401.4 | Reverse | TGTTCCTCGGGTCCTACAGA | Â | Â |
Glut4 | Forward | TCACTAGATCCCGGAGAGCC | 35 | 235 |
NM_001359114.1 | Reverse | GGGTTCCCCATCCTTACGTC | Â | Â |
GAPDH | Forward | CCCTTAAGAGGGATGCTGCC | 30 | 124 |
NM_001289726.1 | Reverse | TACGGCCAAATCCGTTCACA | Â | Â |
β-actin | Forward | GATTACTGCTCTGGCTCCTAG | 30 | 147 |
NM_007393.5 | Reverse | ACTCATCGTACTCCTGCTTG | Â | Â |