Fig. 1
From: Pathogenic Cav3.2 channel mutation in a child with primary generalized epilepsy
Biophysical effects of Cav3.2 mutations. a Schematic representation of the Cav3.2 channel showing the approximate location of the R1282W and A1765V mutations. b Representative Ba2+ current traces recorded from WT, R1282W and A1765V channels. c Average current densities (pA/pF) as a function of voltage for WT, R1282W and A1765V channels, showing a 2-fold increase in current densities of the R1282W mutant. d Steady-state inactivation curves for WT, R1282W and A1765V channels. e Representative western blot of surface biotinylation of WT and R1282W channels showing no difference in surface expression. Beads control represents non-biotinylated cells. Mock represents mock transfected cells. The graph shows quantified Cav3.2 integrated density normalized by Na+K+ATPase signal