Fig. 3

pS25 and pS1201 are spatiotemporally regulated in mouse developing brain. a Immunohistochemistry of E15 mouse brain sagittal section using pan-MAP 1B, pS25 and pS1201 Abs, respectively. Scale bar: 500 μm. b, c and d Immunofluorescent staining of E15 neocortex using the Abs against cell adhesion molecule L1 (red), as axonal marker [21]; and pS25 or pS1201 (green). The neuronal cell nuclei were recognized using DAPI (blue). Although expression of MAP 1B were detected in cortical plate and ventricular zone, the staining of pS25 and pS1201 Abs was mainly restricted to the intermediate zone, similar to L1. Scale bar: 50 μm. e The expression patterns of MAP 1B, pS25, pS1201 and SMI-31 at developmental stages and in adult mouse brain, evaluated by western blotting. f Quantitative assessment of blot intensity showed higher phosphorylation level at a perinatal stage than in adulthood. Data are shown as mean ± SD; n = 3; * p < 0.05, ** p < 0.01, *** p < 0.001 by one-way ANOVA with Dunnett’s multiple comparisons test