Fig. 4

Immunohistochemistry of various brain regions in E15 mouse, using pS25 and pS1201 Abs. Microscopic images of sagittal sections derived from various regions were DAB-stained using pS25 (a, b, e-j) or pS1201 Abs (c, d, k-p). Boxes in (a-d) represent the regions enlarged in (e-p), respectively. Both pS25 and pS1201 Abs succeeded in labelling the bundles of nerve fibers, such as internal capsule, lateral olfactory tract (a, c), optic chiasm (b, d), anterior commissure (e, k), fimbria of hippocampus, and stria medullaris (g, m). In the neocortex, the intermediate zone, where the developing axons are enriched, was specifically labelled (F, L). Fibers in striatum (e, k), dorsal thalamus (h, n), superior colliculus (i, o), and cerebellum (j, p) were also labelled. The scale bar: 500 μm (c, d; in a-d), 200 μm (p; in e-p), respectively. Abbreviations: AC, anterior commissure; CB, cerebellum; CP, cortical plate; DTH, dorsal thalamus; EGL, external granular layer; FI, fimbria of hippocampus; IC, internal capsule; IZ, intermediate zone; LO, lateral olfactory tract; MED, medulla; OCH, optic chiasm; PK, Purkinje cell layer; PON, pons; SC, superior colliculus; SM, stria medullaris; STR, striatum