Fig. 1
From: Peripheral nerve injury-induced alterations in VTA neuron firing properties
Electrophysiological characterization of lateral and medial VTA DA neurons. a Spontaneous firing frequencies in the lateral and medial VTA. b-f Biophysical properties including membrane potential (b), leak current (c), capacitance (d), input resistance (e), and action potential threshold (f) of medial and lateral VTA DA neurons. h Current clamp stimulation protocols for measuring the F-I relation (upper) and Ih (lower). Scale bar, 50 mV, 100 ms. i Representative current clamp traces showing AP firing patterns at 100 pA from lateral (upper) and medial (lower) VTA DA neurons. Scale bar, 20 mV, 100 ms. j Representative current clamp traces showing activation of Ih in lateral (upper) and medial (lower) VTA DA neurons. The red dashed lines indicate where peak response and steady-state were measured for calculation of the voltage sag. Scale bar, 10 mV, 100 ms. k Average F-I relation curves for medial and lateral VTA DA neurons. l F-I slope for medial and lateral VTA DA neurons. m Ih amplitude recorded from medial and lateral VTA DA neurons. Statistics, Mann-Whitney test. **, p < 0.01; ****, p < 0.0001. Numbers in parentheses reflect numbers of cells and animals, and are presented as (N = cells/N = animals)