Fig. 3

Immunofluorescence (IF) analysis of the hippocampal GFAP and IBA1 expression in CA1 Hipp region and orexin A positive cell numbers in LH of the WT and A53T mice. Representative IF microphotographs of the DAPI, p-α-syn, GFAP, IBA1 and merged image in 5-mo WT mice (a, b) and A53T mice (c, d). Representative high-magnification (20x) IF images (b, d) were used for densitometry analysis. Representative low-magnification (10x) IF images of orexin field (e) and high magnification images of orexin neurons (f, 63x, oil). Image J was used to quantify the intensity of GFAP and IBA1 staining and density of IBA1 positive cells. The A53T mice showed an increased expression of GFAP (g) and IBA1 (h) compared to WT mice. The A53T mice showed increased density of IBA1 positive cells (i) and no change in orexin A positive cell numbers (j). (n = 5/group; Student’s T test; **p < 0.01)