Fig. 1
From: Postsynaptic density protein 95 (PSD-95) is transported by KIF5 to dendritic regions
Expression of KIF5A ΔMD mutant reduces the number and average size of PSD-95 particles in dendrites. Cultured rat hippocampal neurons were infected with Sindbis viruses encoding GFP, GFP-KIF5A (WT), or GFP-KIF5A (ΔMD), incubated for 9 h, and immunostained with monoclonal anti-PSD-95 antibody. a Representative images of infected neurons. Scale bar: 20 μm. b ΔMD expression significantly reduces the number of PSD-95 particles (GFP: 100.00% ± 3.88%, n = 31 dendrites, 3417 μm; KIF5A [WT]: 111.70% ± 4.91%, n = 31 dendrites, 3724 μm; KIF5A [ΔMD]: 78.48% ± 4.23%, n = 29 dendrites, 3287 μm; Kruskal–Wallis test: P < 0.0001; Dunn’s multiple comparison test: ** P < 0.01, ns: not significant). c ΔMD expression significantly reduces the average size of PSD-95 particles (GFP: 100.00% ± 5.83%, n = 31 dendrites; KIF5A [WT]: 88.39% ± 4.27%, n = 31 dendrites; KIF5A [ΔMD]: 61.71% ± 4.00%, n = 29 dendrites; Kruskal–Wallis test: P < 0.0001; Dunn’s multiple comparison test: *** P < 0.001, ns: not significant). N values denote the dendritic number from n neurons