Fig. 2
From: Postsynaptic density protein 95 (PSD-95) is transported by KIF5 to dendritic regions
PSD-95 is colocalized with KIF5. a Representative images of immunostaining. Scale bar: 20 μm. Cultured neurons were immunostained with polyclonal anti-PSD-95 antibody and monoclonal anti-KIF5 antibody (H2), followed by Alexa Fluor® 488 anti-rabbit IgG antibody for PSD-95 and Cy3-conjugated anti-mouse IgG antibody for KIF5. b Results of co-localization analysis. 53.17% ± 3.86% (n = 19 dendrites, 1354 μm) of KIF5-immunopositve puncta are colocalized with PSD-95-immunopositve puncta and 62.55% ± 1.69% (n = 19 dendrites, 1354 μm) of PSD-95-immunopositive puncta are colocalized with KIF5-immunopositve puncta. c Results of proximity ligation assay. Cultured neurons were infected with Sindbis viruses encoding GFP and subjected to PLA. Red dots in PLA indicate an interaction between the two proteins. Scale bar: 20 μm. d Results of IP assays using rat brain lysates. In total 500 μg of rat brain lysate was used in the IP assays using 3 μg monoclonal anti-KIF5 antibodies or polyclonal PSD-95 antibodies. These were analyzed by Western blotting using the antibody indicated. Asterisks indicate interaction bands in the Western blot assays