Fig. 3
From: Postsynaptic density protein 95 (PSD-95) is transported by KIF5 to dendritic regions
The PDZ3 domain of PSD-95 and the tail region of KIF5A are required for the interaction. Full-length PSD-95 or truncated mutants with full-length KIF5A (a, c), and full-length KIF5A or truncated mutants with full-length PSD-95 (b, d) were transfected into HEK 293T cells. The cell lysates were then used in immunoprecipitation assays. a Truncated mutants of PSD-95 and interaction with full-length KIF5A, analyzed by co-IP assays. GK: Guanosine monophosphate kinase. b Truncated mutants of KIF5A and interaction with full-length PSD-95, analyzed by co-IP assays. c, d Results of co-IP assays. The lysates were precipitated with anti-FLAG antibody, and the immunoprecipitates were analyzed in Western blotting assays using anti-HA antibody