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Fig. 5 | Molecular Brain

Fig. 5

From: Postsynaptic density protein 95 (PSD-95) is transported by KIF5 to dendritic regions

Fig. 5

ADPDZ3 expression reduces the level of PSD-95 in dendrites. Cultured rat hippocampal neurons were infected with Sindbis viruses encoding GFP or GFP-ADPDZ3 and incubated for 9 h to allow expression. The cultures were then subjected to immunostaining using monoclonal anti-PSD-antibody and Cy3-conjugated anti-mouse IgG antibody or polyclonal anti-synapsin I antibody and Alexa Fluor® 647 anti-rabbit IgG antibody. They were then visualized using confocal microscopy. a Representative images of expressed neurons. Arrows indicate analyzed dendrites. Scale bar: 20 μm. b, c ADPDZ3 expression reduced the number of PSD-95 particles in the dendrites (GFP: 100.00% ± 6.08%, n = 32, 1766 μm; ADPDZ3: 82.27% ± 4.03%, n = 35, 1973 μm; * P < 0.05), but not that of synapsin I particles (GFP: 100.00% ± 6.00%, n = 32, 1766 μm; ADPDZ3: 98.36% ± 5.41%, n = 35, 1973 μm; ns: not significant). Scale bar: 20 μm. d ADPDZ3 expression significantly reduced colocalization between PSD-95 and synapsin I (GFP: 0.350 ± 0.037, n = 32; ADPDZ3: 0.214 ± 0.035, n = 37; ** P < 0.01). N values indicate number of dendrites from multiple neurons derived from three different batches

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