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Fig. 6 | Molecular Brain

Fig. 6

From: Postsynaptic density protein 95 (PSD-95) is transported by KIF5 to dendritic regions

Fig. 6

ADPDZ3 expression reduces surface glutamate receptor 1 (GluA1) in dendrites. Cultured rat hippocampal neurons were infected with Sindbis viruses encoding GFP or GFP-ADPDZ3 and incubated for 9 h to allow expression. The cultures were then subjected to immunostaining using polyclonal GluA1 antibody and Cy3-conjugated anti-mouse IgG antibody. They were visualized using confocal microscopy. a, b Representative images of expressed neurons and selected dendrites in the analyses. Dotted boxes indicate analyzed dendrites. Scale bar: 20 μm. c, d ADPDZ3 expression reduced the number of surface GluA1 particles (GFP: 100.00% ± 7.83%, n = 18, 1486 μm; ADPDZ3: 66.50% ± 6.21%, n = 14, 1254 μm; ** P < 0.01), but not size of the surface GluA1 particles (GFP: 100.00% ± 4.51%, n = 18; ADPDZ3: 110.80% ± 6.82%, n = 14, ns: not significant). N values indicate number of dendrites from multiple neurons derived from three different batches

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