Fig. 1

Transcriptome analyses of the mPFC in homozygous wild-type (WT) and Fkbp5 knock-out (KO) mice following chronic stress. a Schematic timeline of the induction of chronic restraint stress. b Effect of CRS on sucrose preference. Data were combined with the 1st and 2nd sucrose preference test (SPT) results. Control mice (CT) n = 9; CRS-exposed WT mice (WT_ST) n = 6; CRS-exposed Fkbp5 KO mice (KO_ST) n = 6. One-way ANOVA (F[2,39] = 11.67, p = 0.0001); Fisher’s LSD: ***p < 0.001. c Multidimensional scaling (MDS) plot for transcriptomes of individual samples of CT (yellow), WT_ST (blue) or KO_ST (red). d The interleaved scatter plots of modules which have a significant negative correlation with stress. Data represent mean ± SEM. One-way ANOVA; Fisher’s LSD: *p ≤ 0.05, **p < 0.01. e A heatmap showing the expression of M55 genes in the mPFC of CT (left), WT_ST (middle) and KO_ST (right). f A network plot of M55 genes and their intramodular connections. The ten hub genes (the top ten genes with highest intramodular connectivity; Cul9, Polm, Ttll8, Vmn1r90, Tacc3, Mir877, Mmp25, Bhlhe23, Wtip and Ube2d-ps) are shown in red. Note their central position in the network, suggesting high intramodular connectivity. g Enrichment dot plot for Gene Ontology (GO) analysis of M55 genes. The 13 GO terms with the lowest p-value each annotation cluster are plotted in order of gene ratio. The dot size represents the number of genes associated with a specific term. The dot color represents the adjusted p-value