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Fig. 3 | Molecular Brain

Fig. 3

From: Differential regulation of Cav2.2 channel exon 37 variants by alternatively spliced μ-opioid receptors

Fig. 3

Biotinylation of Cav2.2e37a/Cavβ1/Cavα2δ-1 in the presence of mMOR1, mMOR1C and mMOR1O.Biotinylated cell surface proteins were isolated and normalized to Na/K-ATPase levels. a Representative blot of Cav2.2-37a surface and total expression (top blots) and Na/K-ATPase surface and total expression (bottom blots). b Quantification of plasma membrane Cav2.2-37a/ Cavβ1/Cavα2δ-1 channel expression in the absence and the presence of mMOR1, mMOR1C or mMOR1O (normalized by Na/K-ATPase cell surface expression). c Quantification of total Cav2.2-37a/Cavβ1/Cavα2δ-1 expression in the absence or the presence of mMOR1, mMOR1C or mMOR1O (normalized by total Na/K-ATPase expression). d Normalized surface to total expression of Cav2.2-37a channels. Data are from 4 independent experiments. n.s. – not significant (Kruskal-Wallis test)

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Molecular Brain

ISSN: 1756-6606