Fig. 4
From: Systematic analysis of expression signatures of neuronal subpopulations in the VTA
Distribution of neuron subpopulations in the VTA. (a) Schematic showing the procedure for selecting the final marker candidates from Slc17a6-, Th-, and Gad1-like genes. See the text for details. (b) Top five alternative marker candidates were selected from Slc17a6- and Th-like genes, and conventional markers were listed with or without a final candidate (red) for each type of neuron. Spearman’s correlations (Rho) are shown, along with their P values. (c) ISH images showing expression of the indicated genes at the single-cell resolution. Axes: D, dorsal; V, ventral; M, medial; L, lateral. (d) Representative IHC images showing expression of the indicated proteins. The images were obtained from M2 and M6 locations in Fig. 2a for visualizing of P2RY14-VGLUT2 labeling and CHRNA6-TH labeling, respectively. (e) Numbers of neurons expressing the indicated proteins (3 mice and 11 brain slices per marker candidate; for Slc17a6 like [M location: 6 images, L location: 6 images], for Th like [M location: 7 images, L location: 7 images] used for analysis). Data are shown as mean ± SEM. Slc17a6-like: VGLUT2+, 77.71 ± 19.03 cells/mm2; P2RY14+, 71.41 ± 16.63 cells/mm2; double-positive (co-local.), 67.21 ± 14.22 cells/mm2; Th-like: TH+, 484.62 ± 26.90 cells/mm2; CHRNA6+, 500.95 ± 29.60 cells/mm2; co-local., 474.48 ± 14.22 cells/mm2