Fig. 6
From: Systematic analysis of expression signatures of neuronal subpopulations in the VTA
Validation of selected marker gene by IHC analysis. (a) Representative IHC images showing expression of the indicated proteins in the medial part of the VTA. The images in the 3rd column show expression of WWOX (green), TH (blue), and VGLUT2 (red) in a subregion indicated by the white dotted box in the merged image. The representative images were obtained from M3 location in Fig. 2a. (b) Mander’s overlap coefficient (MOC) analysis was performed on IHC images. Pixel-based overlapped coefficients between green and blue (G:B), green and red (G:R), and red and blue (R:B) channels, n = 10 cells. (c) Pie charts showing the proportions of WWOX expressing neurons expressing or not expressing TH and/or VGLUT2 in the medial (left) and lateral (right) parts of the VTA (6 mice and 22 brain slices, M location: 22 images, L location: 14 images were used for analysis). (d) Representative images showing medial and lateral distributions of neurons expressing WWOX (green), TH (blue) and VGLUT2 (red). These images were obtained from M2 and L1 locations in Fig. 2a. (e) Bar graphs showing the numbers of neurons expressing the indicated proteins in the medial (M) and lateral (L) regions of the VTA (image data same as c). Data are shown as mean ± SEM. M: TH+, 359.17 ± 28.98 cells/mm2; VGLUT2+, 224.48 ± 14.89 cells/mm2; WWOX+, 197.31 ± 16.10 cells/mm2; L: TH+, 407.78 ± 18.02 cells/mm2; VGLUT2+, 112.07 ± 5.77 cells/mm2; WWOX+, 70.21 ± 6.08 cells/mm2