Fig. 3
Genetic interactions between Fmi and dTrim9 in C4da topography. a, c Representative images of the dorsal-ventral view of single C4da terminals (green) and the C4da neuropil marked by ppk-tdTomato (red). b Overexpression of dTrim9 causes the axon terminals of M neurons to shift to the ventral side, mixing with the axon terminals of V neurons, resulting in a defect in topography, but this phenotype disappears in the fmi mutant. c The statistic results of axon terminals TI of three neurons of C4da. Overexpression of Fmi has no effects on the phenotype of the dTrim9 mutation. OE, overexpress; −/−, null allele of mutation; D, ddaC; M, v’ada; V, vdaB. *P < 0.05; **P < 0.01; N.S., no significant difference. Scale bar, 2 μm. Error bars indicate mean ± SEM. WT: D, n = 24; M, n = 22; V, n = 23; OE dTrim9: D, n = 24; M, n = 23; V, n = 21; OE dTrim9 + fmiE59: D, n = 26; M, n = 22; V, n = 23; dTrim9−/−: D, n = 23; M, n = 20; V, n = 23; dTrim9−/− + OE Fmi: D, n = 25; M,n = 29; V, n = 26