Fig. 1
From: Delayed expression of activity-dependent gating switch in synaptic AMPARs at a central synapse
Delayed expression of activity-dependent acceleration in the kinetics of eEPSCs. a Schematic diagram shows details of experimental paradigm for induction and expression phases in cell-attached configuration before establishing whole-cell recording mode to measure eEPSCs (or mEPSCs) at different time points (Δt) after theta burst stimulation (TBS). Low frequency stimulation (LFS, 0.05 Hz) was given throughout experiments except for the period of TBS application. Parameters for TBS are given in the box. Control experiments were performed in contralateral MNTB nuclei of the same slices (naĂ¯ve) where cells experienced no TBS prior to membrane rupture. b Examples of whole-cell recordings of averaged eEPSCs from naĂ¯ve (middle panel) and TBS (left panel) synapses at Δt = 45 min, for which the decay phase is fit with a double exponential curve function with respective fast and slow time constants given (Ï„fast and Ï„slow). Scaled eEPSCs from naive and TBS synapses are superimposed to illustrate the accelerated time course of synaptic response by TBS (right panel). c Averaged eEPSC amplitude of naive and TBS synapses (left panel) or another control group which experiences 1 h of LFS at 0.05 Hz without TBS (right panel). d Averaged eEPSC Ƭfast and Ƭslow values from naĂ¯ve, TBS and LFS control synapses. e-f Plots summarizing time dependent changes in Ƭfast and amplitude of eEPSCs after TBS compared to naĂ¯ve controls. There are significant differences (p < 0.05) in both parameters between 15 and 45 min in TBS group but not naĂ¯ve control group. g Paired pulse ratio (PPR) at intervals of 3.3, 5 and 10 ms are plotted for naĂ¯ve and TBS synapses. Holding potential was − 60 mV for this and subsequent figures. Statistical analysis is performed between neuron populations with unpaired t-tests with significance denoted as * p < 0.05