Fig. 5

The AMPAR gating switch requires synergistic Ca²⁺ signaling to Ca²⁺ dependent kinases and protein synthesis. a-b Example recordings of raw and scaled mEPSCs from slices pretreated with EGTA-AM (a) or BAPTA-AM (b) followed by co-application of 100 μM NMDA + 100 μM DHPG show that BAPTA but not EGTA blocks the increase in the size of A_0.4 of mEPSCs. c-e Summary plots showing PKC inhibitor (10 nM Go6983) (c) or CamKII blocker (15 μM KN-62) (d) or protein synthesis inhibitor (10 μM cyclohexamide) (e) blocks TBS-induced increase in the size of A_0.4 component of mEPSCs. Note that cycloheximide increased the amplitude of mEPSCs in TBS treated synapses but not naïve synapses