Fig. 6

Sodium currents in DRG neurons expressing CRMP2 and its mutants. a Representative traces recorded from a non-transfected DRG neuron (control), of the total sodium current recorded from DRG neurons expressing CRMP2-WT-GFP or CRMP2-K374A-GFP without exposure to TNF-α and after being exposed to TNFα for 6 h. b-f comparisons of the current density-voltage relationships for the total sodium current recorded from control, CRMP2-WT and CRMP2-K374A neurons. For panels (b) and (c), the control group is compared to CRMP2-WT and CRMP2-K374A groups, respectively. For panels (d) and (e), the CRMP2-K374A group is compared to CRMP2-WT and CRMP2-K374A + TNF-α groups, respectively. For panel (f), all previous groups are depicted, and the CRMP2-WT + TNFα group is added. § control vs CRMP2 WT + TNFα; * control vs CRMP2-K374A; @ CRMP2 WT vs CRMP2-K374A; # CRMP2-K374A vs CRMP2-K374A + TNFα. §, *, @ and #, p < 0.05; Two Way ANOVA test followed by Bonferroni. g shows the current density peak values measured at − 5 mV for all of the studied groups. *, # and §, p < 0.05; One Way ANOVA test followed by Bonferroni. n reflects numbers of cells. + TNF reflects 6 h TNF-α exposure