Fig. 3

Reduced single-cell motility in an MLC1-expressing cell. a Representative images from a wound healing assay of COS-7 cells expressing wildtype MLC1 and patient-derived mutants (P92S- and S280 L-GFP). COS-7 cells expressing GFP-tagged proteins were visualized by 488 nm filter set. Scale bar: 200 μm. b Quantification of wound healing activity ((width of initial wound–width of final wound)/24 h) of cells expressing GFP (n = 19), MLC1-GFP (n = 22), and patient-derived mutants (P92S-GFP (n = 16) and S280 L-GFP (n = 17)). c Representative snapshots and trajectories of single-cell level live cell imaging. Scale bar: 50 μm. Live cell imaging was used to analyze single-cell motility in COS-7 cells expressing GFP (n = 27), MLC1-GFP (n = 40), and patient-derived mutants (P92S-GFP, n = 19 and S280 L-GFP, n = 10). Trajectory (d), mean velocity (e), and directionality (f) were analyzed as described in the Methods section. Lower directionality scores are indicative of more random movement