Fig. 6
From: Plasma membrane localization of MLC1 regulates cellular morphology and motility
Downregulation of Mlc1 induced lamellipodia formation and dynamic membrane fluctuation in primary astrocytes. Primary astrocytes infected with rAAV encoding GFP and shScrambled Mlc1 (shScr, a) or shMlc1 (b) were stained with anti-GFP (green, shRNA-transfected), Arp3 (cyan), and phalloidin (magenta) antibodies. In the magnified cell-cell contact points (bottom), fibrous actin bundles and branched actin networks are indicated by empty and filled arrowheads, respectively. Scale bar: 20 μm (upper) and 5 μm (bottom). c Time-lapse imaging of primary astrocytes expressing GFP-LifeAct and shScr or shMlc1. Scale bar: 25 μm