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Fig. 1 | Molecular Brain

Fig. 1

From: An RNAi screen for secreted factors and cell-surface players in coordinating neuron and glia development in Drosophila

Fig. 1

A transgenic RNAi screen targeting secreted proteins and cell-surface receptors in the developing visual system. A-A” and B-B″, 3rd-instar larval eye-brain complexes were double-stained to visualize the expression pattern of UAS-mCD8-GFP under control of the eye-specific ey3.5-GAL4 or WG-specific Mz97-GAL4 drivers. A-A”, 3rd-instar larval eye-brain complexes carrying UAS-mCD8-GFP under control of ey3.5-GAL4 were labeled with GFP fluorescence (green) and anti-Bdl staining (magenta). ey3.5-GAL4 drove the expression of target genes in all cell types in the epithelial monolayer of eye disc (ed), but not in WG. B-B″, 3rd-instar larval eye-brain complexes carrying UAS-mCD8-GFP under control of Mz97-GAL4 were labeled with GFP fluorescence (green) and MAb24B10 staining (magenta). Mz97-GAL4 drove gene expression in WG as well as some other glial types in the optic lobe, but not in the epithelial monolayer of eye disc. C, A scheme of the RNAi screen. UAS-RNAi transgenes were simultaneously expressed in the eye disc and sub-retinal WG under control of both ey3.5-GAL4 and Mz97-GAL4. Abbreviations: ed., eye disc; ol, optic lobe; os, optic stalk. Scale bar: 20 μm

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