Fig. 3
Knockdown affected WG development only. UAS-RNAi transgenes were expressed in both R cells and WG under control of ey3.5-GAL4 and Mz97-GAL4. 3rd-instar larval eye-brain complexes were double-stained with MAb24B10 (green) and anti-Bdl (magenta). A-A”, wild type. B-B″, an eye-brain complex in which the RNAi line (i.e. BDSC# 28624) targeting alrm was expressed in both eye disc and WG. In knockdown animals, although R cells in the eye disc developed normally (B and B”), some WG processes failed to cease extension in the lamina, and instead projected aberrantly into the deeper medulla (arrows) (B’). C-C″, an alrm-like WG extension phenotype was observed in animals expressing the RNAi line (BDSC# 34661) targeting Tsp. Scale bar: 20 μm