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Fig. 4 | Molecular Brain

Fig. 4

From: An RNAi screen for secreted factors and cell-surface players in coordinating neuron and glia development in Drosophila

Fig. 4

Knockdown affected both R-cell and WG development. UAS-RNAi transgenes were driven by both ey3.5-GAL4 and Mz97-GAL4. 3rd-instar larval eye-brain complexes were double-stained with MAb24B10 (green) and anti-Bdl (magenta). A-A”, wild type. B-B″, an eye-brain complex in which the RNAi line (i.e. BDSC# 33642) targeting mys was expressed in the eye disc and WG. While the differentiation of R cells and WG appeared normal, the projection pattern of R-cell axons and WG processes was severely disrupted. Both R-cell axons and WG processes stalled at the posterior end of the eye disc (arrow in B and B’) and failed to enter the optic stalk. C-C″, the organization of R cells and WG was disrupted in animals in which the RNAi line (i.e. BDSC# 38207) targeting shg was expressed in the eye disc and WG. D-D”, an eye-brain complex in which the RNAi line (i.e. BDSC# 25781) targeting EGFR was expressed in the eye disc and WG. EGFR knockdown decreased the number of differentiating R cells and WG. E-E”, expression of the RNAi line (i.e. BDSC# 37496) targeting nrg also disrupted the differentiation of R cells and WG. F-F″, expression of the RNAi line (i.e. BDSC# 33416) targeting ama completely blocked the differentiation of R cells and WG. Abbreviations: ed., eye disc; ol, optic lobe; os, optic stalk. Scale bar: 20 μm

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