Fig. 3

Enumeration of the hair cells in surface preparations of cochleae at P30. a, b Phalloidin staining and immunostaining with anti-Myo7a of the hair cells at basal turns in the CONT (left) and TSK-KO mice (right). c The numbers of outer and inner hair cells in the CONT and TSK-KO mice were not significantly different; p = 0.09, 0.30, and 0.18, respectively, for apical, middle, and basal outer hair cells; p = 0.46, 0.29, and 0.45, respectively, for apical, middle, and basal inner hair cells. d, e Scanning electron micrographs of the hair cells in the CONT and TSK-KO mice. f Length of stereocilia in the inner hair cells in the CONT and TSK-KO mice. Stereocilia in the inner hair cells were significantly shortened and dislocated in the TSK-KO mice. ****, p < 0.0001 at all turns. IHC: inner hair cell, OHC: outer hair cell, N.S.: not significant, WT: wild-type