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Fig. 1 | Molecular Brain

Fig. 1

From: Diverged morphology changes of astrocytic and neuronal primary cilia under reactive insults

Fig. 1

Arl13b-positive and AC3-positive primary cilia of Arl mice are longer than those of FVB/N and C57BL/6 wildtype mice. a-c, g Arl mice had longer Arl13b-positive primary cilia than control mice in the hippocampal CA1, CA3, and DG regions. a Arl mice, b FVB/N controls, c C57BL/6 controls. Representative images were taken from the CA1 region. Scale bar, 5 μm. g Comparison of Arl13b + cilia length in the hippocampal CA1, CA3, and DG of three mouse strains. Arl mice, N = 8; FVB/N mice, N = 5; C57BL/6, N = 4. Cilia number: Arl mice: 399, 230, and 435; FVB/N: 213, 146, and 254; C57BL/6: 144, 118, and 116. Data were compared with one-way ANOVA test with post hoc Tukey test. CA1: F(2, 753) =307, p < 0.001; CA3: F(2, 491) = 251, p < 0.001; DG: F(2, 802) = 241, p < 0.001. (d-f, h) Arl mice had longer AC3-positive primary cilia in three subregions in the hippocampus. d Arl mice, e FVB/N controls, f C57BL/6 controls. Scale bar, 5 μm. h Comparison of AC3+ primary cilia length in hippocampal CA1, CA3, and DG regions. Arl mice, N = 4; FVB/N, N = 5; C57BL/6, N = 3. Cilia number: Arl mice, 403, 471, and 527; FVB/N mice: 386, 343, and 555; C57BL/6: 202, 422, and 180. Data were analyzed with one-way ANOVA test with post hoc Tukey test. CA1: F(2, 988) = 58, p < 0.001; CA3: F(2, 1233) = 40, p < 0.001; DG: (2, 1259) = 21, p < 0.001. i CDFs and histogram density of cilia length show the distribution differences of Arl13b-positive cilia length in different hippocampal regions of the three mouse strains. j CDFs and histogram density of cilia length demonstrate the distribution differences of AC3-positive cilia length in different regions of three mouse strains. k Ratios of Arl13b- and AC3-positive cilia length in Arl mice relative to those of C57BL/6 mice. Cilia length ratios for Arl13b-positive primary cilia: CA1: 2.1; CA3: 2.1; and DG: 1.9; Cilia length ratios for AC3-positive primary cilia: CA1: 1.4; CA3: 1.2; and DG: 1.1. For (a-k), cilia length data were collected from 4 to 8 months old mice. l-m Primary cultures of astrocytes from Arl mice had much longer Arl13b-positive primary cilia than those from C57BL/6 mice. l Arl13b-positive cilia in primarily cultured astrocytes were significantly longer than those derived from C57BL/6 mice (***, p < 0.001, unpaired Student’s t-test). Data were collected from 6 Arl cultures, 5 C57BL/6 cultures. Cilia number: Arl mice, 50; C57BL/6 mice, 57. m CDFs and density comparisons demonstrate the differences in Arl13b-positive cilia length between Arl mice and C57BL/6 mice. n AC3-positive cilia in primarily cultured cortical neurons (~ 10 days in vitro) were significantly longer than those derived from C57BL/6 mice (***, p < 0.001, unpaired Student’s t-test). Data were collected from 6 Arl cultures, 6 C57BL/6 cultures. Cilia number: Arl mice, 87; C57BL/6 mice, 87. o CDFs and density comparisons demonstrate the differences in AC3-positive cilia length between Arl mice and C57BL/6 mice

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