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Fig. 5 | Molecular Brain

Fig. 5

From: Diverged morphology changes of astrocytic and neuronal primary cilia under reactive insults

Fig. 5

Naturally occurring seizures reduce the length of AC3-positive and Arl13b-positive primary cilia in the hippocampus. a-b Arl13b-positive cilia in the hippocampal CA1 and DG regions of Arl seizure mice (b) were shorter than those of non-seizure Arl control mice (a). Scale bar: 5 μm. c The length of Arl13b-positive cilia in the CA1 and DG regions, but not in the CA3 region, was significantly shorter in Arl seizure mice than in Arl non-seizure mice (***, p < 0.001, unpaired Student’s t-test). Arl non-seizure controls, N = 4; Arl seizure mice, N = 5. Cilia number: Non-seizure mice: 160, 130, and 253; Seizure mice: 180, 131, and 183. d CDFs and histogram density comparison present the length of Arl13b-positive cilia in the CA1, CA3, and DG regions. e-f AC3-positive cilia in the CA3 region of Arl non-seizure control mice (e) and Arl seizure mice (f). Scale: 5 μm. g Cilia length comparison revealed the shortening of AC3-positive cilia in three hippocampal regions of seizure mice (CA1: *, p < 0.05, CA3: ***, p < 0.001, DG: *, p < 0.05, unpaired Student’s t-test). Non-seizure controls, N = 4; seizure mice, N = 5 animals. Cilia number: non-seizure mice: 403, 471, and 527; seizure mice: 374, 294, and 375. h CDFs and histogram density show AC3-positive cilia length in the hippocampal CA1, CA3, and DG regions. i Seizure does not significantly affect the percent of Arl13b-positive cilia in the DG. Data collected from 3 non-seizure and 3 seizure Arl mice. Cell number: control: 185; seizure: 196. Cilia number: control: 64; seizure: 51. n.s. not significant, with unpaired Student’s t-test. j Seizure does not significantly affect the percent of AC3-positive cilia in the DG. Data collected from 3 non-seizure and 3 seizure Arl mice. Cell number: control: 292; seizure: 414. Cilia number: control: 221; seizure: 282. n.s. not significant, with unpaired Student’s t-test. k Centrin2-GFP expression in hippocampal regions in Arl seizure mice had no significant difference with that in Arl non-seizure mice. Centrin2 imaging density over 50 μm2 regions per mouse was calculated. Data were collected from 3 non-seizure and 3 seizure Arl mice. p = 0.6, 0.9, and 0.5 respectively with unpaired Student’s t-test

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